Human IL-7 R alpha / CD127 Protein (I66T, V138I), Mouse IgG2a Fc Tag

Interleukin-7 receptor subunit alpha (IL7Ra) is also known as CD antigen CD127, which belongs to the type I cytokine receptor family and type 4 subfamily. IL7Ra /CD127 contains 1 fibronectin type-III domain. IL7Ra /CD127 is expressed on various cell types, including naive and memory T cells and many others. IL7Ra /CD127 is receptor for interleukin-7 and also acts as a receptor for thymic stromal lymphopoietin (TSLP) . The IL7 receptor is a heterodimer of IL7R and IL2RG. The TSLP receptor is a heterodimer of CRLF2 and IL7R.

Product Specifications

Background

Specifications

This protein carries a mouse IgG2a Fc tag at the C-terminus. The protein has a calculated MW of 52.0 kDa. The protein migrates as 75-95 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Accession Number

P16871-1 (I66T, V138I)

Expression Region

Glu 21 - Gly 236

Host

HEK293

Target

IL-7 R alpha

Conjugation

Unconjugated

Tag

C-mFc (mIgG2a)

Source

Human

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

90%

Bioactivity

Immobilized Human IL-7 Protein, premium grade (Cat. No. IL7-H4219) at 2 μg/mL (100 μL/well) can bind Human IL-7 R alpha, Mouse IgG2a Fc Tag (Cat. No. IL7-H5258) with a linear range of 4-63 ng/mL (QC tested).