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Rabbit IgG Fc Protein, Tag Free (MALS verified)

Rabbit IgG Fc, Tag Free (IGG-R5203) is expressed from human 293 cells (HEK293) . It contains AA Cys 105 - Lys 323 (Accession # P01870-1) .

Product Specifications

Background

Crystallizable fragments composed of the carboxy-terminal halves of both IMMUNOGLOBULIN HEAVY CHAINS linked to each other by disulfide bonds. Fc fragments contain the carboxy-terminal parts of the heavy chain constant regions that are responsible for the effector functions of an immunoglobulin (COMPLEMENT fixation, binding to the cell membrane via FC RECEPTORS, and placental transport) .

Specifications

This protein carries no "tag". The protein has a calculated MW of 24.8 kDa. The protein migrates as 28-32 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Accession Number

P01870-1

Expression Region

Cys 105 - Lys 323

Host

HEK293

Target

IgG Fc

Conjugation

Unconjugated

Tag

Native

Source

Rabbit

Stability

● -20°C to -70°C for 12 months in lyophilized state; ● -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

95%

Bioactivity

Immobilized Rabbit IgG Fc, Tag Free (Cat. No. IGG-R5203) at 5 μg/mL (100 μL/well) can bind Biotinylated Human CD64, His,Avitag (Cat. No. FCA-H82E8) with a linear range of 0.3-5 ng/mL (QC tested).

Format

Powder

Buffer

50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5

Additives

Trehalose

Molecular Weight

24.8 kDa

Shipping Conditions

RT

Storage Conditions

-20°C

Package Size

200ug*1

Product Datasheet

Https://www.acrobiosystems.com/static/main/products/pdf/ds/IGG-R5203.pdf

Species

Rabbit

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