Human IgG3 Fc Protein, Tag Free (MALS & SPR verified)
Human IgG3 Fc, Tag Free (IG3-H5200) is expressed from human 293 cells (HEK293) . It contains AA Glu 99 - Lys 377 (Accession # P01860) .
Product Specifications
Background
Immunoglobulin G3 (IgG3) is a member of many immunoglobulin G developed and secreted by effective B cells. In wake of cutting by pepsin, IgG is divided into two F (ab) s with one antigen binding site and a high conserved Fc segment. The Fc segment bears a highly conserved N-glycosylation site. Ig gamma-3 chain C region (IgG3-Fc / IGHG3) contains two constant regions of IgG3 H chain (CH2, CH3) .
Specifications
This protein carries no "tag". The protein has a calculated MW of 31.2 kDa. The protein migrates as 35-42 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Accession Number
P01860
Expression Region
Glu 99 - Lys 377
Host
HEK293
Target
IgG3 Fc
Conjugation
Unconjugated
Tag
Native
Source
Human
Stability
● -20°C to -70°C for 12 months in lyophilized state; ● -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Endotoxin
1.0 EU per μg
Purity
95%
Format
Powder
Buffer
Tris with Glycine, Arginine and NaCl, pH7.5
Additives
Trehalose
Molecular Weight
31.2 kDa
Shipping Conditions
RT
Storage Conditions
-20°C
Package Size
500ug*2
Product Datasheet
Https://www.acrobiosystems.com/static/main/products/pdf/ds/IG3-H5200.pdf
Species
Human
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