Human NKG2C&CD94 Protein, Fc Tag

Human NKG2C&CD94, Fc Tag (NC4-H5258) is expressed from human 293 cells (HEK293) . It contains AA Ile 94 - Leu 231 (NKG2C) & Asp 57 - Ile 179 (CD94) (Accession # P26717-1 (NKG2C) & Q13241-1 (CD94) (F102S, mutation caused by sequence update) ) .

Product Specifications

Background

CD94 plays a role as a receptor for the recognition of MHC class I HLA-E molecules by NK cells and some cytotoxic T-cells. KLRD1 (CD94) is an antigen preferentially expressed on NK cells and is classified as a type II membrane protein because it has an external C terminus. NKG2C/CD159c is used as a receptor for NK cells and some cytotoxic T cells to recognize MHC class I HLA-E molecules. CD94 pairs with the NKG2 molecule as a heterodimer. The CD94/NKG2 complex, on the surface of natural killer cells interacts with Human Leukocyte Antigen (HLA) -E on target cells.

Specifications

This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 57.1 kDa. The protein migrates as 65-80 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Accession Number

P26717-1 (NKG2C) & Q13241-1 (CD94)

Expression Region

Ile 94 - Leu 231 (NKG2C) & Asp 57 - Ile 179 (CD94)

Host

HEK293

Target

NKG2C & CD94

Conjugation

Unconjugated

Tag

C-Fc

Source

Human

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

95%

Bioactivity

Immobilized Human NKG2C&CD94, Fc Tag (Cat. No. NC4-H5258) at 5 μg/mL (100 μL/well) can bind PE-Labeled Human HLA-E*01:03&B2M&CMV UL40 (VMAPRTVLL) Tetramer Protein (Cat. No. HLU-HP2H5) with a linear range of 0.01-5 μg/mL (QC tested).