Human Frizzled-5 / FZD5 Protein, Fc Tag

Frizzled-5 (FZD5) is also known as FzE5, which belongs to the G-protein coupled receptor Fz/Smo family. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. FZD5 contains one FZ (frizzled) domain. FZD5 may be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. FZD5 interacts specifically with Wnt5A to induce the beta-catenin pathway. FZD5 interacts with GOPC.

Product Specifications

Background

Specifications

This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 42.6 kDa. The protein migrates as 50-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Accession Number

Q13467

Expression Region

Ala 27 - Pro 167

Host

HEK293

Target

Frizzled-5

Conjugation

Unconjugated

Tag

C-Fc

Source

Human

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

95%

Bioactivity

Immobilized Human Frizzled-5, Fc Tag (Cat. No. FZ5-H5259) at 1 μg/mL (100 μL/well) can bind Wnt surrogate Human IgG1(D239E,L241M) | Human Lambda with a linear range of 0.1-8 ng/mL (QC tested).