Human Frizzled-2 / FZD2 Protein, Fc Tag

Frizzled-2 (FZD2) is also known as FzE2, which belongs to the G-protein coupled receptor Fz/Smo family. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. FZD2 contains one FZ (frizzled) domain. FZD2 may be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. The Lys-Thr-X-X-X-Trp motif of FZD2 interacts with the PDZ doman of Dvl (Disheveled) family members and is involved in the activation of the Wnt/beta-catenin signaling pathway.

Product Specifications

Background

Specifications

This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 41.7 kDa. The protein migrates as 50-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Accession Number

AAI13403

Expression Region

Gln 24 - Ser 156

Host

HEK293

Target

Frizzled-2

Conjugation

Unconjugated

Tag

C-Fc

Source

Human

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

95%

Bioactivity

Immobilized Human Frizzled-2, Fc Tag (Cat. No. FZ2-H5251) at 1 μg/mL (100 μL/well) can bind Wnt surrogate Human IgG1(D239E,L241M) | Human Lambda with a linear range of 0.1-8 ng/mL (QC tested).