Human NKG2D / CD314 Protein, Mouse IgG2a Fc Tag

NKG2D is a transmembrane protein belonging to the CD94/NKG2 family of C-type lectin-like receptors, also known as KLRK1, CD314, D12S2489E, KLR and killer cell lectin like receptor K1. NKG2D itself forms a homodimer whose ectodomains serve for ligand binding. NKG2D is a major recognition receptor for the detection and elimination of transformed and infected cells as its ligands are induced during cellular stress, either as a result of infection or genomic stress such as in cancer. In NK cells, NKG2D serves as an activating receptor, which itself is able to trigger cytotoxicity. The function of NKG2D on CD8+ T cells is to send co-stimulatory signals to activate them.

Product Specifications

Background

Specifications

This protein carries a mouse IgG2a Fc tag at the N-terminus. The protein has a calculated MW of 43.5 kDa. The protein migrates as 50-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Accession Number

P26718-1

Expression Region

Ile 73 - Val 216

Host

HEK293

Target

NKG2D

Conjugation

Unconjugated

Tag

N-mFc (mIgG2a)

Source

Human

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

0.1 EU per μg

Purity

95%

Bioactivity

Immobilized Human MICA, His Tag (Cat. No. MIA-H5221) at 1 μg/mL (100 μL/well) can bind Human NKG2D, Mouse IgG2a Fc Tag (Cat. No. NKD-H5259) with a linear range of 0.02-0.313 μg/mL (QC tested).