Human CD79A&CD79B Heterodimer Protein, Mouse IgG2a Fc, Flag Tag&Mouse IgG2a Fc, His Tag (MALS verified)

CD79a and CD79b heterodimers are the important signaling components of B cell receptor (BCR) complex which plays a crucial role in B cell development and antibody production. BCR complexes are composed of a ligand-binding receptor (membrane immunoglobulin; mIg), non-covalently associated with the signaling com-ponent, a disulfide-linked heterodimer of CD79a (Igα) and CD79b (Igβ) . Both CD79 subunits consist of an Ig-like domain, a transmembrane (TM) region, and a longer cytoplasmic tail (CYT) containing an immune-receptor tyrosine-based activation motif (ITAM) .

Product Specifications

Background

Specifications

Human CD79A&CD79B Heterodimer Protein, Mouse IgG2a Fc, Flag Tag&Mouse IgG2a Fc, His Tag is produced by co-expression of CD79A and CD79B, has a calculated MW of 43.8 kDa (CD79A) and 46.7 kDa (CD79B) . Subunit CD79A carries a mouse IgG2a Fc fragment at the C-terminus, followed by a flag tag and subunit CD79B carries a mouse IgG2a Fc fragment at the C-terminus, followed by a polyhistidine tag. The protein migrates as 120 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.

Accession Number

P11912-1 (CD79A) & P40259-1 (CD79B)

Expression Region

Leu 33 - Arg 143 (CD79A) & Ala 29 - Asp 159 (CD79B)

Host

HEK293

Target

CD79A & CD79B

Conjugation

Unconjugated

Tag

C-mFc (mIgG2a) & C-Flag | C-mFc (mIgG2a) & C-10xHis

Source

Human

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

0.1 EU per μg

Purity

95%

Bioactivity

Immobilized Human CD79A&CD79B Heterodimer Protein, Mouse IgG2a Fc,Flag Tag&Mouse IgG2a Fc,His Tag (Cat. No. CDB-H52W3) at 1 μg/mL (100 μL/well) can bind Polatuzumab with a linear range of 0.1-1 ng/mL (QC tested).