Human CD44 Protein, His Tag

CD44 antigen is a cell-surface glycoprotein involved in cell–cell interactions, cell adhesion and migration. CD44 is expressed in a large number of mammalian cell types. CD44 is a receptor for hyaluronic acid and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs) . CD44 function is controlled by its posttranslational modifications. One critical modification involves discrete sialofucosylations rendering the selectin-binding glycoform of CD44 called HCELL (for Hematopoietic Cell E-selectin/L-selectin Ligand) . CD44 participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing, hematopoiesis, and tumor metastasis. Transcripts for this gene undergo complex alternative splicing that results in many functionally distinct isoforms.

Product Specifications

Background

Specifications

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 23.3 kDa. The protein migrates as 32-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Accession Number

AAH04372

Expression Region

Gln 21 - Pro 220

Host

HEK293

Target

CD44

Conjugation

Unconjugated

Tag

C-9xHis

Source

Human

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

90%

Bioactivity

Immobilized Human CD44, His Tag (Cat. No. CD4-H5226) at 5 μg/mL (100 μL/well) can bind Hyaluronan biotin sodium salt with a linear range of 39-156 ng/mL (Routinely tested).