Unconjugated Human TPBG / 5T4 Protein, His, Avitag™ (MALS verified)

Trophoblast glycoprotein (TPBG), also known as 5T4, is the therapeutic target of several anticancer agents currently in clinical development, largely due to its high expression in tumors and low expression in normal adult tissues.This gene encodes a leucine-rich transmembrane glycoprotein that may be involved in cell adhesion. TPBG is expressed by all types of trophoblasts as early as 9 weeks of development.

Product Specifications

Background

Specifications

This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™) . The protein has a calculated MW of 39.0 kDa. The protein migrates as 50-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Accession Number

Q13641-1

Expression Region

Ser 32 - Ser 355

Host

HEK293

Target

TPBG

Conjugation

Unconjugated

Tag

C-10xHis & C-Avi

Source

Human

Applications

TPG-H52E5 is the unconjugated version of TPG-H82Eb (Biotinylated Human TPBG, His, Avitag) . It can be utilized for assay development or customized biotinylation according to the experimental designs.

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

0.1 EU per μg

Purity

90%

Bioactivity

Immobilized Unconjugated Human TPBG, His,Avitag (Cat. No. TPG-H52E5) at 1 μg/mL (100 μL/well) can bind Anti-TPBG Antibody, Human IgG1 with a linear range of 0.1-2 ng/mL (QC tested).

Format

Powder

Buffer

PBS, pH7.4

Additives

Trehalose

Molecular Weight

39.0 kDa

Additionnal Information

Please see 'Shipping-and-Payments' sheet. Website: https://www.acrobiosystems.com/support/shipping-and-payments

Shipping Conditions

Storage Conditions

-20°C

Package Size

50ug*1

Host or Source

HEK293

Species

Human

Protein ID

Q13641-1

Preservative

Trehalose

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