Human LRRC32 / GARP / Garpin Protein, Fc Tag

Leucine-rich repeat protein 32 (LRRC32), also known as GARP (glycoprotein A repetitions predominant) . LRRC32 expression promotes the acquisition of a Treg phenotype including reduced cellular proliferation, reduced cytokine secretion, and the capacity to suppress the proliferation of naïve T cells. LRRC32 binds directly to the TGF-beta latency associated peptide (LAP) and tethers latent TGF-beta on the surface of activated Treg cells. The presentation of TGF-beta on Tregs contributes to their ability to suppress naïve T cell proliferation.

Product Specifications

Background

Specifications

This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 92.4 kDa. The protein migrates as 105 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Accession Number

Q14392-1

Expression Region

His 20 - Asn 627

Host

HEK293

Target

LRRC32

Conjugation

Unconjugated

Tag

C-Fc

Source

Human

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

95%

Bioactivity

Immobilized Human LRRC32, Fc Tag (Cat. No. LR2-H5256) at 2 μg/mL (100 μL/well) can bind Cynomolgus Latent TGF-Beta 1 (C33S), His Tag (Cat. No. TG1-C5243) with a linear range of 0.039-1.25 μg/mL (Routinely tested).