Human LIGHT / TNFSF14 Protein, Mouse IgG2a Fc Tag, low endotoxin

Tumor necrosis factor ligand superfamily member 14 (LIGHT) is a tumor necrosis factor (TNF) superfamily ligand that regulates T cell immune responses by signaling through the herpes virus entry mediator (HVEM) and the lymphotoxin beta receptor (LTbetaR) . LIGHT has emerged as a potent initiator of T cell co-stimulation signals effecting CTL-mediated tumor rejection, allograft rejection and graft versus host disease. Constitutive expression of LIGHT leads to tissue destruction and autoimmune-like disease syndromes.

Product Specifications

Background

Specifications

This protein carries a mouse IgG2a Fc tag at the N-terminus. The protein has a calculated MW of 45.1 kDa. The protein migrates as 50 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Accession Number

O43557-1

Expression Region

Asp 74 - Val 240

Host

HEK293

Target

LIGHT

Conjugation

Unconjugated

Tag

N-mFc (mIgG2a)

Source

Human

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

0.01 EU per μg

Purity

95%

Bioactivity

Immobilized Human HVEM, His Tag (Cat. No. HVM-H52E9) at 5 μg/mL (100 μL/well) can bind Human LIGHT Protein, Mouse IgG2a Fc Tag (Cat. No. LIT-H5256) with a linear range of 0.3-5 ng/mL (QC tested).