Biotinylated Human TACI / TNFRSF13B Protein, Fc, Avitag™

Transmembrane activator and CAML interactor (TACI), also known as tumor necrosis factor receptor superfamily member 13B (TNFRSF13B) . It was originally discovered because of its ability to interact with calcium-modulator and cyclophilin ligand (CAML) . TACI was later found to play a crucial role in humoral immunity by interacting with two members of the TNF family: BAFF and APRIL. The present study demonstrated that, in NSCLC, a proliferation-inducing ligand (APRIL), B-cell maturation antigen (BCMA) and transmembrane activator and CAML interactor (TACI) proteins are abnormally expressed by immunohistochemistry, reverse transcription-quantitative polymerase chain reaction and western blotting. In addition, the expression of APRIL, BCMA and TACI were observed to be involved in extracellular signal-regulated kinase (ERK) 1/2 activation in A549 cells.

Product Specifications

Background

Specifications

The protein has a calculated MW of 46.7 kDa. The protein migrates as 33-40 kDa and 47-50 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation and furin cleave site.

Accession Number

O14836-1

Expression Region

Ser 2 - Thr 166

Host

HEK293

Target

TACI

Conjugation

Biotin-labeled

Tag

C-Fc & C-Avi

Source

Human

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

90%

Bioactivity

Immobilized Human BAFF, Fc Tag, active trimer (Cat. No. BAF-H5261) at 1 μg/mL (100 μL/well) can bind Biotinylated Human TACI, Fc,Avitag (Cat. No. TAI-H82F6) with a linear range of 2-25 ng/mL (QC tested).