Human TNFSF11 / RANKL / CD254 Protein, Fc Tag

Receptor activator of nuclear factor kappa-B ligand (RANKL), also known as tumor necrosis factor ligand superfamily member 11 (TNFSF11), TNF-related activation-induced cytokine (TRANCE), osteoprotegerin ligand (OPGL), and osteoclast differentiation factor (ODF), is known as a type II membrane protein and is a member of the tumor necrosis factor (TNF) superfamily. RANKL, through its ability to stimulate osteoclast formation and activity, is a critical mediator of bone resorption and overall bone density. Some findings also suggestion some cancer cells, particularly prostate cancer cells, can activate an increase in bone remodeling and ultimately increase overall bone production.[17] This increase in bone remodeling and bone production increases the overall growth of bone metastasizes. The overall control of bone remodeling is regulated by the binding of RANKL with its receptor or its decoy receptor, respectively, RANK and OPG.

Product Specifications

Background

Specifications

This protein carries a human IgG1 Fc tag at the N-terminus. The protein has a calculated MW of 46.9 kDa. The protein migrates as 50-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Accession Number

AAC51762.1

Expression Region

Gly 64 - Asp 245

Host

HEK293

Target

TNFSF11

Conjugation

Unconjugated

Tag

N-Fc

Source

Human

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

90%

Bioactivity

Immobilized Human RANK, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. RAK-H5251) at 0.5 μg/mL (100 μL/well) can bind Human TNFSF11, Fc Tag (Cat. No. RAL-H5265) with a linear range of 0.1-3 ng/mL (QC tested).