Mouse Neuropilin-1 / NRP1 / CD304 Protein, Mouse IgG2a Fc Tag, low endotoxin

Neuropilin-1 (NRP1) is also known as Vascular endothelial cell growth factor 165 receptor (VEGF165R), CD antigen CD304, which belongs to the neuropilin family. The membrane-bound isoform 1 is a receptor involved in the development of the cardiovascular system, in angiogenesis, in the formation of certain neuronal circuits and in organogenesis outside the nervous system. It mediates the chemorepulsant activity of semaphorins. It binds to semaphorin 3A, The PLGF-2 isoform of PGF, The VEGF-165 isoform of VEGF and VEGF-B. Coexpression with KDR results in increased VEGF-165 binding to KDR as well as increased chemotaxis. It may regulate VEGF-induced angiogenesis. The soluble isoform 2 binds VEGF-165 and appears to inhibit its binding to cells.

Product Specifications

Background

Specifications

This protein carries a mouse IgG2a Fc tag at the C-terminus. The protein has a calculated MW of 120.5 kDa. The protein migrates as 130-180 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Accession Number

P97333-1

Expression Region

Phe 22 - Pro 856

Host

HEK293

Target

Neuropilin-1

Conjugation

Unconjugated

Tag

C-mFc (mIgG2a)

Source

Mouse

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

0.1 EU per μg

Purity

90%

Bioactivity

Immobilized Human VEGF165, premium grade (Cat. No. VE5-H4210) at 2 μg/mL (100 μL/well) can bind Mouse Neuropilin-1 Protein, Mouse IgG2a Fc Tag (Cat. No. NR1-M5254) with a linear range of 0.5-4 ng/mL (QC tested).