Human Podoplanin / PDPN Protein, His Tag (SPR verified)

Podoplanin (PDPN) is a type I transmembrane mucin-like glycoprotein expressed in the lymphatic endothelium. It is overexpressed in several solid tumors, such as squamous cell carcinoma, malignant mesothelioma, Kaposi sarcoma, angiosarcoma, testicular seminoma, and brain tumors. PDPN expression is associated with malignant progression, epithelial–mesenchymal transition, and metastasis.

Product Specifications

Background

Specifications

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 13.0 kDa. The protein migrates as 20 kDa and 27-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Accession Number

Q86YL7-1

Expression Region

Ala 23 - Leu 131

Host

HEK293

Target

Podoplanin

Conjugation

Unconjugated

Tag

C-10xHis

Source

Human

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

0.1 EU per μg

Purity

90%

Bioactivity

Immobilized Human Podoplanin, His Tag (Cat. No. PON-H52H3) at 5 μg/mL (100 μL/well) can bind Human CLEC-2, Fc Tag (Cat. No. CL2-H5254) with a linear range of 0.313-5 μg/mL (QC tested).