Human NFL Protein, His Tag

Human NFL, His Tag (NFL-H5143) is expressed from E. coli cells. It contains AA Ser 2 - Asp 543 (Accession # P07196-1).

Product Specifications

Background

Neurofilaments (NF) are classed as type IV intermediate filaments found in the cytoplasm of neurons. Together with microtubules and microfilaments, they form the neuronal cytoskeleton. Neurofilaments usually contain three intermediate filament proteins: NFL, NFM, and NFH which are involved in the maintenance of neuronal caliber. Neurofilament heavy (NFH) can server as biomarkers for several diseases by indicating axonal injury.

Specifications

This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 63.4 kDa.

Expression Region

Ser 2 - Asp 543

Host

E. coli

Target

NFL

Conjugation

Unconjugated

Tag

N-10×His

Source

Human

Stability

The product MUST be stored at -70°C or lower upon receipt; -70°C for 12 months under sterile conditions.

Endotoxin

1.0 EU per μg

Purity

90%

Bioactivity

Immobilized Human NFL, His Tag (Cat. No. NFL-H5143) at 1 μg/mL (100 μL/well) can bind Anti NEFL Antibody with a linear range of 1-16 ng/mL (QC tested).