PE-Labeled Human CD5 Protein, His Tag (Site-specific conjugation)

PE-Labeled Human CD5, His Tag (CD5-HP2H6) is produced via site-specific conjugation of PE to Human CD5, His Tag under optimal conditions with a proprietary technology. Human CD5, His Tag is expressed from human 293 cells (HEK293) . It contains AA Arg 25 - Pro 372 (Accession # P06127-1) .

Product Specifications

Background

T-cell surface glycoprotein CD5 is also known as Lymphocyte antigen T1/Leu-1 and LEU1, which is phosphorylated on tyrosine residues by LYN, so CD5 can create binding sites for PTPN6/SHP-1.CD5 may act as a receptor in regulating T-cell proliferation. CD5 is expressed at various developmental and activation stages on human B cells.CD5 is a well established negative regulator of TCR and BCR signalling.CD5-positive cells may also prevent the emergence of autoimmunity by provision of cytokines such as IL-10. Development, selection and function of different B- and T-cell subsets or their preferential survival may be directly or indirectly dependent on different glycan structures associated with CD5 or CD5-like molecules.

Specifications

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 42.2 kDa.

Accession Number

P06127-1

Expression Region

Arg 25 - Pro 372

Host

HEK293

Target

CD5

Conjugation

PE Labeled

Tag

C-10xHis

Source

Human

Applications

Evaluation of anti-CD5 CAR expression by flow cytometry. Please note that this product is NOT compatible to streptavidin detection system.

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.