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Human CA125 / MUC16 (13810-14451) Protein, His Tag (MALS verified)

The CA125, also known as the MUC16, is a mucin protein that may be found in type I transmembrane or secreted forms that are used monitor the progress of epithelial ovarian cancer therapy. The CA 125 molecule is almost certainly a glycoprotein with a predominance of O-linkages. It is heterogeneous with regard to both size and charge, most likely due to continuous deglycosylation of side chains during its life-span in bodily fluids. It exists as a very large complex (perhaps as much as 4 million daltons) under natural conditions.

Product Specifications

Background

The CA125, also known as the MUC16, is a mucin protein that may be found in type I transmembrane or secreted forms that are used monitor the progress of epithelial ovarian cancer therapy. The CA 125 molecule is almost certainly a glycoprotein with a predominance of O-linkages. It is heterogeneous with regard to both size and charge, most likely due to continuous deglycosylation of side chains during its life-span in bodily fluids. It exists as a very large complex (perhaps as much as 4 million daltons) under natural conditions.

Specifications

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 73.7 kDa. The protein migrates as 90-130 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Accession Number

Q8WXI7-1

Expression Region

Pro 13810 - Pro 14451

Host

HEK293

Target

CA125

Conjugation

Unconjugated

Tag

C-10xHis

Source

Human

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

0.1 EU per μg

Purity

95%

Bioactivity

Immobilized Human CA125 (13810-14451), His Tag (Cat. No. CA5-H52H6) at 5 μg/mL (100 μL/well) can bind Human Mesothelin (296-580), Fc Tag (Cat. No. MSN-H5253) with a linear range of 0.2-10 ng/mL (QC tested).

Format

Powder

Buffer

PBS, pH7.4

Additives

Trehalose

Molecular Weight

73.7 kDa

Additionnal Information

Please see 'Shipping-and-Payments' sheet. Website: https://www.acrobiosystems.com/support/shipping-and-payments

Shipping Conditions

RT

Storage Conditions

-20°C

Package Size

100ug*1

Product Datasheet

https://console.acrobiosystems.com/public/product/pdf/ds/CA5-H52H6.pdf

Host or Source

HEK293

Species

Human

Protein ID

Q8WXI7-1

Preservative

Trehalose

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