PE-Labeled Human EGFRvIII Protein, His Tag (Site-specific conjugation)

PE-Labeled Human EGFRvIII, His Tag (EGI-HP2E3) is produced via site-specific conjugation of PE to Human EGFRvIII, His Tag under optimal conditions with a proprietary technology. Human EGFRvIII, His Tag is expressed from human 293 cells (HEK293) . It contains AA Leu 25 - Ser 378 (Accession # NP_001333870.1) .

Product Specifications

Background

The epidermal growth factor receptor (EGFR; ErbB-1; HER1 in humans) is the cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands. The epidermal growth factor receptor is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinases: EGFR (ErbB-1), HER2/c-neu (ErbB-2), Her 3 (ErbB-3) and Her 4 (ErbB-4) . Mutations affecting EGFR expression or activity could result in cancer. The type III EGF deletion mutant receptor (EGFRvIII) is the most common mutation and was first identified in primary human glioblastoma tumors. This tumor-specific antigen is ligand-independent, contains a constitutively active tyrosine kinase domain, and has been shown to be present in a number of human malignancies. EGFRvIII has been selected as a target for CAR-modified T-cell studies in recent years.

Specifications

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 42.2 kDa.

Accession Number

NP_001333870.1

Expression Region

Leu 25 - Ser 378

Host

HEK293

Target

EGFRvIII

Conjugation

PE Labeled

Tag

C-10xHis

Source

Human

Applications

Please note that this product is NOT compatible to streptavidin detection system.

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.