Biotinylated Human CA125 / MUC16 (13810-14451) Protein, Fc, Avitag™

The CA125, also known as the MUC16, is a mucin protein that may be found in type I transmembrane or secreted forms that are used monitor the progress of epithelial ovarian cancer therapy. The CA 125 molecule is almost certainly a glycoprotein with a predominance of O-linkages. It is heterogeneous with regard to both size and charge, most likely due to continuous deglycosylation of side chains during its life-span in bodily fluids. It exists as a very large complex (perhaps as much as 4 million daltons) under natural conditions.

Product Specifications

Background

Specifications

This protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag™) . The protein has a calculated MW of 100.0 kDa. The protein migrates as 120-160 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Accession Number

Q8WXI7-1

Expression Region

Pro 13810 - Pro 14451

Host

HEK293

Target

CA125

Conjugation

Biotin-labeled

Tag

C-Fc & C-Avi

Source

Human

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

0.1 EU per μg

Purity

90%

Bioactivity

Immobilized Human Mesothelin (296-580), Fc Tag (Cat. No. MSN-H526x) at 5 μg/mL (100 μL/well) can bind Biotinylated Human CA125 (13810-14451), Fc,Avitag (Cat. No. CA5-H82F8) with a linear range of 0.6-78 ng/mL (QC tested).