Cynomolgus CRTAM Protein, Fc Tag

Class I-restricted T cell-associated molecule (CRTAM), a member of nectin family and the immunoglobulin superfamily, is also known as cytotoxic and regulatory T-cell molecule, which is expressed by activated CD8+ and NK T cells. CRTAM is found in spleen, thymus, small intestine, peripheral blood, and surprisingly, in brain where it is highly expressed by Purkinje cells of the cerebellum. The high affinity of CRTAM/IGSF4 adhesion allows CRTAM to disrupt IGSF4 homotypic interactions (3 - 5) . IGSF4 and T cell receptor co-engagement of CRTAM-expressing CD8+ cells induces increased IFN-gamma or IL-22 production (3, 4) . Furthermore, a role in cancer surveillance through NK cell-mediated rejection of IGSF4-expressing tumors has been proposed.

Product Specifications

Background

Specifications

This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 56.6 kDa. The protein migrates as 66-95 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Accession Number

XP_005580021.1

Expression Region

Ser 18 - Gly 287

Host

HEK293

Target

CRTAM

Conjugation

Unconjugated

Tag

C-Fc

Source

Cynomolgus

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

95%

Bioactivity

Immobilized Human CADM1, His Tag (Cat. No. CA1-H5225) at 5 μg/mL (100 μL/well) can bind Cynomolgus CRTAM, Fc Tag (Cat. No. CRM-C5255) with a linear range of 0.6-10 ng/mL (QC tested).