Biotinylated Human PD-L1 / B7-H1 Protein, Fc, Avitag™, His Tag (MALS verified)

Programmed cell death 1 ligand 1 (PDL1) is also known as B7-H, B7H1, MGC142294, MGC142296, PD-L1, PDCD1L1 and PDCD1LG1, which is a member of the growing B7 family of immune molecules and is involved in the regulation of cellular and humoral immune responses.PDL1 is a cell surface immunoglobulin superfamily with two Ig-like domains within the extracellular region and a short cytoplasmic domain. This protein is broadly expressed in the majority of peripheral tissues as well as hematopoietic cells. Interaction between PDL1 and its receptors belonging to the CD28 family of molecules provide both stimulatory and inhibitory signals in regulating T cell activation and tolerance. PDL1 may inhibit ongoing T-cell responses by inducing apoptosis and arresting cell-cycle progression.

Product Specifications

Background

Specifications

This protein carries a human IgG1 Fc fragment at the C-terminus, followed by a polyhistidine tag. The Avi tag (Avitag™) is inserted in-between the Fc and his tags. The protein has a calculated MW of 54.0 kDa. The protein migrates as 65-80 kDa on a SDS-PAGE gel when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition due to glycosylation.

Accession Number

NP_054862.1

Expression Region

Phe 19 - Arg 238

Host

HEK293

Target

PD-L1

Conjugation

Biotin-labeled

Tag

C-Fc & C-Avi & C-6xHis

Source

Human

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

95%

Bioactivity

Immobilized Human PD-1, Fc Tag, low endotoxin (HPLC-verified) (Cat. No. PD1-H5257) at 2 μg/mL (100 μL/well) can bind Biotinylated Human PD-L1, Fc,Avitag,His Tag (Cat. No. PD1-H82F3) with a linear range of 2-39 ng/mL (QC tested).