Human IFN-γ FCM Kit (Flow Cytometry Assay)

The biology and function of IFN-γ has been extensively reviewed in the literature. Cytometric Bead Array (CBA) assays provide a method of capturing a soluble analyte or set of analytes with beads of known size and fluorescence, making it possible to detect analytes using flow cytometry.

Human IFN-γ Kit (Flow Cytometry Beads Assay) is a bead-based immunoassay capable of measuring human interferon-γ (IFN-γ) in serum, plasma, and cell culture supernatant samples.

The unopened kit is stable for 18 months from the date of manufacture if stored at 2°C to 8°C. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

This assay kit is used to measure the concentrations of Human Interferon-γ (IFN-γ) by employing a standard sandwich-FCM format. The antibody encapsulated 1-plex beads, protein (IFN-γ) in specimens and biotin-conjugated detection antibody formed a sandwich complex as bead-analyte-detection antibody. SA-PE was added in and react with biotinylated detection antibody, the intensity of PE fluorescence, in proportion to the recombinant protein titer in specimens, was assessed by flow cytometry at wavelength of 575 nm approximately. The intensity of APC fluorescence was applied to classify bead populations, at wavelength of 670 nm approximately.