ResDetect™ RNase Activity Assay Kit (Fluorescence)

RNases are a class of hydrolytic enzymes that catalyzes both the in vivo and in vitro degradation of ribonucleic acid (RNA) molecules into smaller components. RNase enzymes are categorized into two groups: exoribonucleases and endoribonucleases. RNases are ubiquitous in the environment, and in some biological materials, they are present in relatively high concentrations. RNases also frequently contaminate common molecular biological reagents such as reaction buffers, enzymes such as reverse transcriptase and RNA polymerase, and buffers for RNA purification and storage. It is often used DEPC and/or heaten to remove RNases from containers in experiments. Since even only minute amounts of RNase contamination would ruin the experiment, it is necessary to evaluate the presence of RNase with the RNase Activity Assay Kit (Fluorescence).

RNase Activity Assay Kit (Fluorescence) is a convenient and sensitive assay tool to test the presence of RNase in buffers, reagents, and other components.

1. Unopened kit should be stored at -25~-15°C upon receiving. 2. Find the expiration date on the outside packaging and do not use reagents past their expiration date. 3. The opened kit should be stored per components table. The shelf life is 3 months from the date of opening.

The RNase Activity Assay Kit (Fluorescence) is based on a fluorophore-labeled RNA substrate. When the sample does not contain RNase activity, the substrate is stable and does not produce a fluorescent signal; when the sample contains RNase activity, the substrate is degraded, resulting in a gradual enhanced fluorescence signal, the rate of increase in fluorescence signal is positively correlated with the number and activity of enzymes. Use a fluorescence microplate reader to measure at the wavelength of ex/em= 490/520 nm to determine whether the sample is contaminated by RNase.