SARS-CoV-2 Spike Trimer Protein, His Tag (BA.2.86/USA/EPI_ISL_18110065) (MALS verified)

SARS-CoV-2 Spike Trimer Protein, His Tag (BA.2.86/USA/EPI_ISL_18110065) (SPN-C524y) is expressed from human 293 cells (HEK293) . It contains AA Val 16 - Pro 1213 (Accession # QHD43416.1 (ins16MPLF, T19I, R21T, LPP24-26Del, A27S, S50L, H69del, V70del, V127F, G142D, Y144del, F157S, R158G, N211del, L212I, V213G, L216F, H245N, A264D, I332V, G339H, K356T, S371F, S373P, S375F, T376A, R403K, D405N, R408S, K417N, N440K, V445H, G446S, N450D, L452W, N460K, S477N, T478K, N481K, V483del, E484K, F486P, Q498R, N501Y, Y505H, E554K, A570V, D614G, P621S, H655Y, I670V, N679K, P681R, N764K, D796Y, S939F, Q954H, N969K, P1143L, R683A, R685A, F817P, A892P, A899P, A942P, K986P, V987P) ) . The spike mutations are identified on the SARS-CoV-2 Omicron variant (Pango lineage: BA.2.86/USA/EPI_ISL_18110065) . The recombinant protein is expressed from human 293 cells (HEK293) with T4 fibritin trimerization motif and a polyhistidine tag at the C-terminus. Proline substitutions (F817P, A892P, A899P, A942P, K986P, V987P) and alanine substitutions (R683A and R685A) are introduced to stabilize the trimeric prefusion state of SARS-CoV-2 S protein and abolish the furin cleavage site, respectively.