Biotinylated Human CD6 Protein, His, Avitag™ (MALS verified)

CD6 is a type 1 transmembrane glycoprotein with three extracellular SRCR domains that functions as a co-stimulatory receptor in T cell activation. Its primary ligand is CD166 (ALCAM), an IgSF member expressed on antigen-presenting cells and other tissues. CD6's long cytoplasmic tail contains tyrosine residues that, upon phosphorylation, recruit signaling molecules like SLP76 and SHP1. The CD6-ALCAM interaction promotes T cell activation and downstream MAPK signaling, influencing proliferation, differentiation, and survival. Importantly, CD6 regulates effector T cell (Teff) activity and trafficking, making it a key checkpoint in autoimmunity. Blocking the CD6-ALCAM pathway selectively inhibits pathogenic Teff cells while preserving Treg function, offering a potential therapeutic strategy for autoimmune diseases.

Product Specifications

Background

Specifications

This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™) . The protein has a calculated MW of 44.2 kDa. The protein migrates as 70-90 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Accession Number

P30203-1

Expression Region

His 18 - Glu 398

Host

HEK293

Target

CD6

Conjugation

Biotin-labeled

Tag

C-10xHis & C-Avi

Source

Human

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

90%

Bioactivity

Immobilized Human ALCAM, His Tag (Cat. No. CD6-H5228) at 5 μg/mL (100 μL/well) can bind Biotinylated Human CD6 Protein, His,Avitag (Cat. No. CD6-H82E3) with a linear range of 0.02-5 μg/mL (QC tested).