HCoV-OC43 Spike protein, His Tag

Human coronavirus OC43 (HCoV-OC43) is one of seven known coronaviruses to infect humans, which is responsible for the common cold and may have been responsible for the 1889–1890 pandemic. The infecting coronavirus is an enveloped, positive-sense, single-stranded RNA virus that enters its host cell by binding to the N-acetyl-9-O-acetylneuraminic acid receptor. The spike protein of HCoV-OC43 is a trimer protruding from the viral membrane to engage cellular receptors and mediate viral fusion with host membranes. Each spike trimer contains two large regions: N-terminal S1 responsible for receptor binding and C-terminal S2 responsible for fusion.

Product Specifications

Background

Specifications

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 148.3 kDa. The protein migrates as 170 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) .

Accession Number

P36334-1

Expression Region

Ala 14 - Pro 1297 (RRSRG 754-758 GGSGG)

Host

Insect Cells

Target

Spike protein

Conjugation

Unconjugated

Tag

C-10xHis

Source

HCoV-OC43

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.