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Biotinylated Human HLA-A*11:01&B2M Monomer Protein (Peptide free, MALS verified)

Biotinylated Human HLA-A*11:01&B2M Monomer Protein (HLM-H82W5) is expressed from human 293 cells (HEK293) . It contains AA Gly 25 - Thr 305 (HLA-A*11:01) & Ile 21 - Met 119 (B2M) (Accession # AAV53343.1 (HLA-A*11:01) & P61769 (B2M) ) .

Product Specifications

Background

Human papillomavirus (HPV) is A kind of papillomavirus belonging to the milk polypoid virus family. It is a spherical DNA virus, which can cause the proliferation of squamous epithelium of human skin mucosa. HPV (human papillomavirus) for common warts, genital warts (condyloma acuminatum), and other symptoms. There are many types of human papillomavirus (HPV), with HPV 16 and 18 being high-risk types known to significantly increase the risk of cervical, vaginal and vulvar cancers in women and men. The PE-labeled human HLA-A*1101 HPV (IVCPICSQK) tetramer protein is a complex of HLA-A*1101 of the MHC Class I, B2M, and IVCPICSQK peptide of the HPV.

Specifications

This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™) . The protein has a calculated MW of 36.0 kDa and 11.7 kDa. The protein migrates as 42-45 kDa and 13 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Accession Number

AAV53343.1 (HLA-A*11:01) & P61769 (B2M)

Expression Region

Gly 25 - Thr 305 (HLA-A*11:01) & Ile 21 - Met 119 (B2M)

Host

HEK293

Target

HLA-A*11:01 & B2M

Conjugation

Biotin-labeled

Tag

C-10xHis & C-Avi

Source

Human

Stability

● -20°C to -70°C for 12 months in lyophilized state; ● -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

95%

Bioactivity

Immobilized Biotinylated Human HLA-A*11:01&B2M Monomer Protein (Cat. No. HLM-H82W5) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Anti-HLA class I Antibody, Human IgG1 (W6/32) with a linear range of 0.00003-0.008 ng/mL (QC tested).

Format

Powder

Buffer

PBS, pH7.4

Additives

Trehalose

Molecular Weight

36.0 kDa and 11.7 kDa

Shipping Conditions

RT

Storage Conditions

-20°C

Package Size

25ug*1

Product Datasheet

Https://www.acrobiosystems.com/static/main/products/pdf/ds/HLM-H82W5.pdf

Species

Human

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