Biotinylated Human HLA-A*03:01&B2M&KRASG12D (VVVGADGVGK) Complex Protein (Monomer, MALS verified)

Biotinylated Human HLA-A*03:01&B2M&KRASG12D (VVVGADGVGK) Complex Protein (HLD-H82E3) is expressed from human 293 cells (HEK293) . It contains AA Gly 25 - Thr 305 (HLA-A*03:01) & Ile 21 - Met 119 (B2M) & VVVGADGVGK peptide (Accession # P04439-1 (HLA-A*03:01) & P61769-1 (B2M) & VVVGADGVGK) .

Product Specifications

Background

The Kirsten rat sarcoma 2 viral oncogene homolog (KRAS) oncogene plays a critical role in the initiation and maintenance of pancreatic tumors and its signaling network represents a major target for therapeutic intervention. The Human HLA-A*0201 KRAS (KLVVVGAGGV) complex protein is a complex of HLA-A*0201 of the MHC Class I, B2M, and KLVVVGAGGV peptide of the KRAS.

Specifications

Biotinylated Human HLA-A*03:01&B2M&KRASG12D (VVVGADGVGK) Complex Protein is produced by co-expression of HLA and B2M loaded with KRASG12D peptide. This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™) . The protein has a calculated MW of 35.9 kDa and 11.7 kDa. The protein migrates as 40-43 kDa and 10 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Accession Number

P04439-1 (HLA-A*03:01) & P61769-1 (B2M) & VVVGADGVGK

Expression Region

Gly 25 - Thr 305 (HLA-A*03:01) & Ile 21 - Met 119 (B2M) & VVVGADGVGK peptide

Host

HEK293

Target

HLA-A*0301 & B2M & KRASG12D (VVVGADGVGK)

Conjugation

Biotin-labeled

Tag

C-10xHis & C-Avi

Source

Human

Stability

● -20°C to -70°C for 12 months in lyophilized state; ● -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

95%

Bioactivity

Immobilized Biotinylated Human HLA-A*03:01&B2M&KRASG12D (VVVGADGVGK) Complex Protein (Cat. No. HLD-H82E3) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Anti-HLA class I Antibody, Human IgG1 (W6/32) with a linear range of 0.1-1 ng/mL (QC tested).