Thiolutin

Thiolutin (Acetopyrrothin) is a sulfur-containing antibiotic, which is a potent inhibitor of bacterial and yeast RNA polymerases. Thiolutin can be produced by Streptomyces. Thiolutin inhibits AMSH (IC50 = 4 μM) and Rpn11 (IC50 = 0.53 μM) . Thiolutin is a dual inhibitor of BRCC36 and the NLRP3 inflammasome, exhibiting anti-inflammatory effects. Thiolutin effectively suppresses the interaction between BRCC36 and HMGCR, leading to the inhibition of HCC growth. Thiolutin attenuates pyroptosis and NLRP3 inflammasome activation. Thiolutin markedly alleviates renal injury and inflammatory process in IgAN. Thiolutin is an anti-angiogenic compound which can ease Doxorubicin-induced cardiotoxicity (DOXIC) [1][2][3][4][5].

Product Specifications

CAS Number

[87-11-6]

Product Name Alternative

Acetopyrrothin

UNSPSC

12352005

Hazard Statement

H300

Target

Antibiotic; Bacterial; Deubiquitinase; DNA/RNA Synthesis; HMG-CoA Reductase (HMGCR) ; NOD-like Receptor (NLR) ; Pyroptosis

Type

Natural Products

Related Pathways

Anti-infection; Apoptosis; Cell Cycle/DNA Damage; Immunology/Inflammation; Metabolic Enzyme/Protease

Applications

Metabolism-protein/nucleotide metabolism

Field of Research

Cancer; Infection; Cardiovascular Disease

Assay Protocol

https://www.medchemexpress.com/thiolutin.html

Concentration

10mM

Purity

99.29

Solubility

DMSO : 6.25 mg/mL (ultrasonic; warming; heat to 60°C)

Smiles

CC(NC1=C2C(N(C)C1=O)=CSS2)=O

Molecular Formula

C8H8N2O2S2

Molecular Weight

228.29

Precautions

H300

References & Citations

[1] Wang H, et al. BRCC36 Deubiquitinates HMGCR to Regulate the Interplay Between Ferroptosis and Pyroptosis. Adv Sci (Weinh) . 2024 Mar;11 (11) :e2304263. |[2]Lauinger L, et al. Thiolutin is a zinc chelator that inhibits the Rpn11 and other JAMM metalloproteases. Nat Chem Biol. 2017 Jul;13 (7) :709-714. |[3]Zhang Y, et al. Thiolutin, a novel NLRP3 inflammasome inhibitor, mitigates IgA nephropathy in mice. Int Immunopharmacol. 2025 Apr 16;152:114440. |[4]Cai W, et al. Thiolutin Alleviates Cardiotoxic Effects of Doxorubicin by Suppressing NLRP3 Inflammasome in the Mouse Model. Cardiovasc Toxicol. 2025 Feb;25 (2) :182-192. |[5]Kebaara BW, Nielsen LE, Nickerson KW, Atkin AL. Determination of mRNA half-lives in Candida albicans using thiolutin as a transcription inhibitor. Genome. 2006 Aug;49 (8) :894-9.