Mouse LIGHT / TNFSF14 Protein, His Tag, active trimer, low endotoxin (MALS verified)

Tumor necrosis factor ligand superfamily member 14 (LIGHT) is a tumor necrosis factor (TNF) superfamily ligand that regulates T cell immune responses by signaling through the herpes virus entry mediator (HVEM) and the lymphotoxin beta receptor (LTbetaR) . LIGHT has emerged as a potent initiator of T cell co-stimulation signals effecting CTL-mediated tumor rejection, allograft rejection and graft versus host disease. Constitutive expression of LIGHT leads to tissue destruction and autoimmune-like disease syndromes.

Product Specifications

Background

Specifications

This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 58.9 kDa. The protein migrates as 55-60 kDa and 65-80 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Accession Number

Q9QYH9-1

Expression Region

Asp 72 - Val 239

Host

HEK293

Target

LIGHT

Conjugation

Unconjugated

Tag

N-10xHis

Source

Mouse

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

90%

Bioactivity

Immobilized Mouse LIGHT Protein, His Tag (Cat. No. LIT-M5243) at 5 μg/mL (100 μL/well) can bind Human LTBR, Fc Tag (Cat. No. LTR-H5259) with a linear range of 0.1-2 ng/mL (QC tested).