Human GAD2 / GAD65 Protein, His Tag
Human GAD2 Protein, His Tag (GA2-H55H3) is expressed from Baculovirus-Insect cells. It contains AA Met 1 - Leu 585 (Accession # Q05329) .
Product Specifications
Background
Glutamate decarboxylase or glutamic acid decarboxylase (GAD) is an enzyme that catalyzes the decarboxylation of glutamate to gamma-aminobutyric acid (GABA), the major inhibitory transmitter in higher brain regions, and putative paracrine hormone in pancreatic islets. GAD uses pyridoxal-phosphate (PLP) as a cofactor.Two molecular forms of GAD (65 kDa and 67 kDa) are highly conserved and both are expressed in the CNS, pancreatic islet cells, testis, oviduct and ovary. The isoforms are regionally distributed cytoplasmically in the brains of rats and mice. GAD65 is an ampiphilic, membrane-anchored protein (585 a.a.), and is responsible for vesicular GABA production. GAD67 is cytoplasmic (594 a.a.), and seems to be responsible for significant cytoplasmic GABA production.
Specifications
This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 67.3 kDa. The protein migrates as 65 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) .
Accession Number
Q05329
Expression Region
Met 1 - Leu 585
Host
Insect Cells
Target
GAD2
Conjugation
Unconjugated
Tag
C-10xHis
Source
Human
Stability
● The product MUST be stored at -70°C or lower upon receipt; ● -70°C for 3 months under sterile conditions.
Endotoxin
1.0 EU per μg
Purity
90%
Format
Liquid
Buffer
50 mM Tris, 150 mM NaCl, pH8.0
Additives
Glycerol
Molecular Weight
67.3 kDa
Shipping Conditions
Dry ice
Storage Conditions
-70°C
Package Size
1mg*1
Product Datasheet
Https://www.acrobiosystems.com/static/main/products/pdf/ds/GA2-H55H3.pdf
Species
Human
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