Acridine Orange (zinc chloride salt)

Acridine Orange (Euchrysine 3RX) zinc chloride salt is a cell-penetrable nucleic acid-selective fluorescent dye. Acridine Orange zinc chloride salt produces orange fluorescence when it binds to ssDNA or RNA, and green fluorescence when it binds to dsDNA (Ex: 488 nM; Em: green fluorescence at 530 nm, orange fluorescence at 640 nm) [1][2][3].

Product Specifications

CAS Number

[10127-02-3]

Product Name Alternative

Euchrysine 3RX

UNSPSC

12171500

Hazard Statement

H341

Target

DNA Stain; Fluorescent Dye; Parasite

Type

Dye Reagents

Related Pathways

Anti-infection; Cell Cycle/DNA Damage; Others

Field of Research

Others

Assay Protocol

https://www.medchemexpress.com/acridine-orange-zinc-chloride-salt.html

Solubility

DMSO : 100 mg/mL (ultrasonic) |H2O : 10 mg/mL (ultrasonic)

Smiles

Cl[Zn]Cl.CN(C)C1=CC=C2C(N=C3C=C(N(C)C)C=CC3=C2)=C1.Cl

Molecular Formula

C17H20Cl3N3Zn

Molecular Weight

438.10

Precautions

H341

References & Citations

[1]McMaster GK, et al. Analysis of single- and double-stranded nucleic acids on polyacrylamide and agarosegels by using glyoxal and acridine orange. Proc Natl Acad Sci U S A. 1977 Nov;74 (11) :4835-8. |[2]Traganos F, et al. Simultaneous staining of ribonucleic and deoxyribonucleic acids in unfixed cells using acridine orange in a flow cytofluorometric system. J Histochem Cytochem. 1977 Jan;25 (1) :46-56. |[3]Byvaltsev VA, et al. Acridine Orange: A Review of Novel Applications for Surgical Cancer Imaging and Therapy. Front Oncol. 2019 Sep 24;9:925. |[4]Wang Q, et al. Substrate stiffness regulates the differentiation profile and functions of osteoclasts via cytoskeletal arrangement. Cell Prolif. 2022 Jan;55 (1) :e13172.