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Anti-BirA (mutated/TurboID) - DyLight® 488 conjugated (40 µg)

Selected Variant

AS20 4440-DL488

Size: 40 µg

Product Specifications

Background

Tagging a protein with TurboID allows studying protein interactions in different types of cells and organs and developmental stages, This is a suitable tool for proximity labelling experiments as described in Mair et al, (2019), Proximity labelling of protein complexes and cell-type-specific organellar proteomes in Arabidopsis enabled by TurboID, Elife , 2019 Sep 19;8:e47864, doi: 10,7554/eLife,47864, This tag has MW of 35 kDa.

CAS Number

9007-83-4

HS Code

38221900

Host

Rabbit

Reactivity

BirA (mutated/TurboID)

Not reactive in

No confirmed exceptions from predicted reactivity are currently known

Immunogen

Recombinant mutated BirA protein from E.coli produced using the following plasmid: TurboID-His6_pET21a, (Plasmid #107177) . Expression was done in a vector that allowed for the generation of an untagged protein (without HIS6tag) .

Clonality

Polyclonal

Applications

Immunofluorescence (IF), Western blot (WB)

Field of Research

BC2/Biotin/BirA/DYKDDDDK/GST/Halo/His/...

Dilution

To be determined by end user.

Purity

Immunogen affinity purified serum, in PBS pH 7.4, conjugated to DyLight® 488.

Format

Liquid in PBS pH 7,4.

Molecular Weight

Depends upon fusion partner

Additionnal Information

DyLight® 488 Amax = 493 nm, Emax = 519 nm. DyLight® is a registered trademark of Thermofisher Inc., and its subsidiaries.

References & Citations

To be added when available. Antibody released in May 2023.

Storage Conditions

Store at 4°C for 12-18 months, A preservative may be added for long time storage up to 2 years. Spin briefly the tube before use.

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