CheKine™ Mirco Cell-Wall Binding Acid Invertase (B-AI) Activity Assay Kit

Invertase (Ivr) catalyzes the irreversible decomposition of sucrose into fructose and glucose, and is one of the key enzymes in sucrose metabolism in higher plants. According to the optimal pH, higher plant Ivr can be divided into acidic invertase (AI) and Neutralinvertase (NI) types. The optimal pH of AI is 3~5. There are two types of AI: soluble AI (S-AI) and cell wall insoluble AI (B-AI) . B-AI exists in the intercellular space and binds to the cell wall, and is mainly involved in sucrose decomposition during extracellular unloading in phloem to maintain the concentration of sucrose between pool sources. CheKine™ Micro Cell-Wall Binding Acid Invertase (B-AI) Activity Assay Kit can detect plant tissues samples. In this kit, B-AI catalyzes sucrose degradation to produce reducing sugar, which further reacts with 3,5-dinitrosalicylic acid to produce brown-red amino compounds with characteristic light absorption at 510 nm, and the rate of increase of light absorption at 510 nm is proportional to B-AI activity within a certain range.

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Storage at 4°C and Keep from light immediately upon receipt. Kit has a storage time of 6 months from receipt. Refer to list of materials supplied for storage conditions of individual components.

• If not assayed immediately, samples can be stored at -80°C for one month.• It is recommended to perform several dilutions of your sample to ensure the readings are within the standard value range.• Fresh samples are necessary for good results. If don't perform the assay at the same time, it's better to complete the Sample Preparation step before storing the samples.