Anti-FLASH Antibody

Rabbit Polyclonal Antibody specific to FLASH (CT)

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

FLICE-associated huge protein

Gene Name

CASP8AP2

Gene ID

9994

NCBI Gene ID

UniProt

Q9UKL3

Accession Number

NP_036247

Cellular Locus

Cytoplasm. Nucleus. Nucleus, PML body. Mitochondrion. Note=Exported from the nucleus to the mitochondria upon FAS activation.

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

Peptide corresponding to aa 1253-1268 of human FLASH (accession no. AF165161). This sequence differs by one amino acid from that of mouse.

Target Antigen

CASP8-associated protein 2

Target

FLASH

Clonality

Polyclonal

Isotype

IgG

Type

Antibody

Applications

ELISA, WB, ICC, IF

Field of Research

Apoptosis

Purification Method

Purified by peptide immuno-affinity chromatography

Concentration

Lot Specific

Dilution

Dilute in PBS or medium which is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

Participates in TNF-alpha-induced blockade of glucocorticoid receptor (GR) transactivation at the nuclear receptor coactivator level, upstream and independently of NF-kappa-B. Suppresses both NCOA2- and NCOA3-induced enhancement of GR transactivation. Involved in TNF-alpha-induced activation of NF-kappa-B via a TRAF2-dependent pathway. Acts as a downstream mediator for CASP8-induced activation of NF-kappa-B. Required for the activation of CASP8 in FAS-mediated apoptosis. Required for histone gene transcription and progression through S phase. {PubMed:12477726, PubMed:15698540, PubMed:17003125, PubMed:17245429}.

Additionnal Information

Immunoblotting: use at 0.5ug/mL. Immunocytochemistry: use at 10ug/mL. Positive control: HeLa cell lysate. These are recommended concentrations. Enduser should determine optimal concentrations for their applications.

Storage Conditions

This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles.

Specificity

This antibody recognizes human and mouse FLASH (220kDa).

Formulation

PBS, pH 7.4.

Buffer pH

pH 7.4

Target Background

A mammalian homologue of CED-4 has been identified and designated FLASH (FLICE-associated huge protein). FLASH is involved in Fas-induced apoptosis. FLASH interacts with the DEDs of caspase-8 and FADD through the DED-like domain of FLASH, resulting in activation of caspase-8. FLASH is expressed in multiple tissues and cell types.