Anti-p53DINP1 Antibody

Cytoplasm, cytosol. Nucleus. Nucleus, PML body. Cytoplasmic vesicle, autophagosome. Note=Shuttles between the nucleus and the cytoplasm, depending on cellular stress conditions, and re-localizes to autophagosomes on autophagy activation.

Antiproliferative and proapoptotic protein involved in cell stress response which acts as a dual regulator of transcription and autophagy. Acts as a positive regulator of autophagy. In response to cellular stress or activation of autophagy, relocates to autophagosomes where it interacts with autophagosome-associated proteins GABARAP, GABARAPL1/L2, MAP1LC3A/B/C and regulates autophagy. Acts as an antioxidant and plays a major role in p53/TP53-driven oxidative stress response. Possesses both a p53/TP53-independent intracellular reactive oxygen species (ROS) regulatory function and a p53/TP53-dependent transcription regulatory function. Positively regulates p53/TP53 and p73/TP73 and stimulates their capacity to induce apoptosis and regulate cell cycle. In response to double-strand DNA breaks, promotes p53/TP53 phosphorylation on 'Ser-46' and subsequent apoptosis. Acts as a tumor suppressor by inducing cell death by an autophagy and caspase-dependent mechanism. Can reduce cell migration by regulating the expression of SPARC. {PubMed:11511362, PubMed:22421968, PubMed:22470510}.

Immunoblotting: use at 0.5-1ug/mL <br><br>IHC: use at 2ug/mL. Detection of p53DINP1 in mouse liver with #56223 at 2ug/mL. <br><br>Immunofluorescence: use at 5-20ugml. Detection of p53DINP1 in human lung tissue lysate with #56223 at (A) 0.5ug/mL and (B) 1ug/mL. Detection of p53DINP1 in human liver with #56223 at 5ug/mL.