Cynomolgus ACE2 / ACEH Protein, His Tag

Cynomolgus ACE2, His Tag (AC2-C52H7) is expressed from human 293 cells (HEK293) . It contains AA Gln 18 - Thr 741 (Accession # A0A2K5X283-1) .

Product Specifications

Background

Angiotensin-converting enzyme 2 (ACE2) is also known as ACEH (ACE homolog), is an integral membrane protein with considerable homologous to ACE, which belongs to the peptidase M2 family. ACE2 is an exopeptidase that catalyses the conversion of angiotensin I to the nonapeptide angiotensin, or the conversion of angiotensin II to angiotensin 1-7. ACE2 may be an important regulator of heart function. In case of human coronaviruses SARS and HCoV-NL63 infections, ACE-2 serve as functional receptor for the spike glycoprotein of both coronaviruses. ACE2 is activated by chloride and fluoride, but not bromide and Inhibited by MLN-4760, cFP_Leu, and EDTA, but not by the ACE inhibitors linosipril, captopril and enalaprilat. ACE2 is active from pH 6 to 9, and the optimum pH is 6.5 in the presence of 1 M NaCl.

Specifications

This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 85.7 kDa. The protein migrates as 90-115 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Accession Number

A0A2K5X283-1

Expression Region

Gln 18 - Thr 741

Host

HEK293

Target

ACE2

Conjugation

Unconjugated

Tag

N-10xHis

Source

Cynomolgus

Stability

● The product MUST be stored at -70°C or lower upon receipt; ● -70°C for 3 months under sterile conditions.

Endotoxin

1.0 EU per μg

Purity

95%

Bioactivity

Immobilized Cynomolgus ACE2, His Tag (Cat. No. AC2-C52H7) at 2 μg/mL (100 μL/well) can bind Biotinylated SARS-CoV-2 S protein RBD, His,Avitag (Cat. No. SPD-C82E9) with a linear range of 2-78 ng/mL (QC tested).