Bromodeoxyuridine (BrdU)
Product Specifications
Background
Bromo-deoxyuridine (BrdU) is a thymidine analog that is used in cell proliferation studies. BrdU in culture is incorporated into the DNA during DNA synthesis. Cellular incorporation of BrdU can be detected by anti-BrdU specific antibodies following membrane permeabilization by flow cytometry or immunohistochemistry. The molecular weight of BrdU is 307.1.
Conjugation
Unconjugated
Type
Reagent
Applications
IHC, IHC
Assay Principle
Prior to BrdU immunostaining to detect proliferating cells it is necessary to label the cells or tissue with BrdU. Labeling can either be done in vitro for cell cultures or performed in vivo for experimental animals. Both protocols are provided.
Stability
See expiration date on vial
Concentration
See vial for Concentration
Form
Provided as 5 x 5 mg in sterile filtered solution in phosphate buffered saline
Precautions
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving Humans or animals.
References & Citations
- M. Caroprese, M.G. Ciliberti, P. De Palo, A. Santillo, A. Sevi, M. Albenzio, 2018, Glucocorticoid effects on sheep peripheral blood mononuclear cell proliferation and cytokine production under in vitro hyperthermia,Journal of Dairy Science, ISSN 0022-0302
Shipping Conditions
Ambient Temperature, freeze upon arrival
Storage Conditions
Product should be stored at -20ºC; Aliquot to avoid freeze/thaw cycles
Functional Analysis
IHC
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