Bromodeoxyuridine (BrdU)

Product Specifications

Background

Bromo-deoxyuridine (BrdU) is a thymidine analog that is used in cell proliferation studies. BrdU in culture is incorporated into the DNA during DNA synthesis. Cellular incorporation of BrdU can be detected by anti-BrdU specific antibodies following membrane permeabilization by flow cytometry or immunohistochemistry. The molecular weight of BrdU is 307.1.

Conjugation

Unconjugated

Type

Reagent

Applications

IHC, IHC

Assay Principle

Prior to BrdU immunostaining to detect proliferating cells it is necessary to label the cells or tissue with BrdU. Labeling can either be done in vitro for cell cultures or performed in vivo for experimental animals. Both protocols are provided.

Stability

See expiration date on vial

Concentration

See vial for Concentration

Form

Provided as 5 x 5 mg in sterile filtered solution in phosphate buffered saline

Precautions

This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving Humans or animals.

References & Citations

- M. Caroprese, M.G. Ciliberti, P. De Palo, A. Santillo, A. Sevi, M. Albenzio, 2018, Glucocorticoid effects on sheep peripheral blood mononuclear cell proliferation and cytokine production under in vitro hyperthermia,Journal of Dairy Science, ISSN 0022-0302