PE-Labeled Human CD4 Protein, His Tag (Site-specific conjugation)

PE-Labeled Human CD4, His Tag (CD4-HP2E3) is produced via site-specific conjugation of PE to Human CD4, His Tag under optimal conditions with a proprietary technology. Human CD4, His Tag is expressed from human 293 cells (HEK293) . It contains AA Lys 26 - Pro 396 (Accession # P01730-1) .

Product Specifications

Background

T-cell surface glycoprotein CD4 is also known as T-cell surface antigen T4/Leu-3. CD4 contains three Ig-like C2-type (immunoglobulin-like) domains and one Ig-like V-type (immunoglobulin-like) domain. CD4 is accessory protein for MHC class-II antigen/T-cell receptor interaction. CD4 induces the aggregation of lipid rafts. CD4 is a primary receptor used by HIV-1 to gain entry into host T cells. HIV infection leads to a progressive reduction of the number of T cells possessing CD4 receptors. Therefore, medical professionals refer to the CD4 count to decide when to begin treatment for HIV-infected patients.

Specifications

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 45.0 kDa.

Accession Number

P01730-1

Expression Region

Lys 26 - Pro 396

Host

HEK293

Target

CD4

Conjugation

PE Labeled

Tag

C-10xHis

Source

Human

Applications

Evaluation of anti-CD4 CAR expression by flow cytometry. Please note that this product is NOT compatible to streptavidin detection system.

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.