Recombinant Pseudomonas aeruginosa Exotoxin A (eta), partial

Product Specifications

Product Name Alternative

(ETA) (NAD (+) --diphthamide ADP-ribosyltransferase) (Pseudomonas exotoxin) (PE)

Abbreviation

Recombinant Pseudomonas aeruginosa eta protein, partial

Gene Name

Eta

UniProt

P11439

Expression Region

413-638aa

Organism

Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

Target Sequence

ALLERNYPTGAEFLGDGGDISFSTRGTQNWTVERLLQAHRQLEERGYVFVGYHGTFLEAAQSIVFGGVRARSQDLDAIWRGFYIAGDPALAYGYAQDQEPDARGRIRNGALLRVYVPRSSLPGFYRTGLTLAAPEAAGEVERLIGHPLPLRLDAITGPEEEGGRLETILGWPLAERTVVIPSAIPTDPRNVGGDLDPSSIPDKEQAISALPDYASQPGKPPREDLK

Tag

N-terminal 6xHis-KSI-tagged

Type

In Stock Protein

Source

E.coli

Field of Research

Microbiology

Relevance

An NAD-dependent ADP-ribosyltransferase (ADPRT) . Catalyzes the transfer of the ADP ribosyl moiety of oxidized NAD (NAD (+) ) onto eukaryotic elongation factor 2 (eEF-2) thus arresting protein synthesis . Has an LD (50) of 65 ng/ml against the human lung epithelial cell line C38 .

Endotoxin

Not test

Purity

Greater than 85% as determined by SDS-PAGE.

Activity

Not Test

Form

Liquid or Lyophilized powder

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Molecular Weight

39.9 kDa

References & Citations

"Newly discovered and characterized antivirulence compounds inhibit bacterial mono-ADP-ribosyltransferase toxins." Turgeon Z., Jorgensen R., Visschedyk D., Edwards P.R., Legree S., McGregor C., Fieldhouse R.J., Mangroo D., Schapira M., Merrill A.R. Antimicrob. Agents Chemother. 55:983-991 (2011)

Storage Conditions

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.