Anti-Heme Oxygenase-1 Antibody

Mouse Monoclonal Antibody specific to Heme Oxygenase-1

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

HO-1, EC 1.14.14.18

Gene Name

HMOX1

NCBI Gene ID

UniProt

P09601

Accession Number

NP_002124.1

Cellular Locus

Microsome, Endoplasmic reticulum membrane, Peripheral membrane protein, Cytoplasmic side

Host

Mouse

Reactivity

Human, Mouse, Bovine

Immunogen

Synthetic peptide corresponding to aa 1-30 of human HO-1.

Target Antigen

Heme oxygenase 1

Target

Heme Oxygenase-1

Clonality

Monoclonal

Isotype

IgG1

Type

Antibody

Applications

WB, ELISA

Field of Research

Enzymes

Purification Method

Purified by Protein G affinity chromatography

Concentration

1.0 mg/mL

Dilution

Dilute in PBS or medium that is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

Heme oxygenase cleaves the heme ring at the alpha methene bridge to form biliverdin. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. Under physiological conditions, the activity of heme oxygenase is highest in the spleen, where senescent erythrocytes are sequestrated and destroyed. Exhibits cytoprotective effects since excess of free heme sensitizes cells to undergo apoptosis.

Additionnal Information

ELISA: use at 1ug/ml with HO-1 on the solid phase. Immunoblotting: use at 0.5-1ug/ml. A band of ~32kDa is detected. Immunofluorescence: use at 5-10ug/ml Positive control: Human cell line lysates such as A431, A549, HeLa, HepG2, Jurkat, or MCF7. These are recommended concentrations. Enduser should determine optimal concentrations for their applications.

Storage Conditions

This antibody is stable for at least one (1) year at -20°C.

Specificity

This antibody recognizes human, mouse, and bovine HO-1. It does not cross-react with HO-2.

Formulation

PBS, pH 7.4.

Buffer pH

pH 7.4

Target Background

Heme-oxygenase is an enzyme that catalyzes heme catabolism to yield biliverdin, iron, and carbon monoxide (CO). There are three isoforms of heme-oxygenase: HO-1, HO-2, and HO-3. HO-1 and HO-2 have been identified as the two major isoforms in mammals. HO-1, also known as heat shock protein 32 (Hsp32) is induced by most oxidative stress inducers, cytokines, inflammatory agents, and heat shock. HO-1 deficiency appears to cause reduced stress defense, a pro-inflammatory tendency, susceptibility to atherosclerotic lesion formation, endothelial cell injury, and growth retardation. Therefore, up-regulation of HO-1 is one of the major defense mechanisms against oxidative stress.