Anti-EndoPDI Antibody

Mouse Monoclonal Antibody specific to EndoPDI

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

Endoplasmic reticulum resident protein 46, ER protein 46, ERp46, Thioredoxin-like protein p46

Gene Name

TXNDC5

NCBI Gene ID

<a href="https://www.ncbi.nlm.nih.gov/gene/?term=TXNDC5">TXNDC5</a>

UniProt

Q8NBS9

Cellular Locus

Endoplasmic reticulum lumen

Host

Mouse

Reactivity

Human

Immunogen

Synthetic peptide corresponding to aa 52-63 (ADGEDGQDPHSK) of human EndoPDI (accession no. NP_001139021).

Target Antigen

Thioredoxin domain-containing protein 5

Target

EndoPDI

Clonality

Monoclonal

Isotype

IgG2a

Type

Antibody

Applications

WB, IHC, ICC/IF, IP, ELISA

Field of Research

Neuroscience

Purification Method

Purified by Protein G affinity chromatography

Concentration

1.0 mg/mL

Dilution

Dilute in PBS or medium that is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

Possesses thioredoxin activity. Has been shown to reduce insulin disulfide bonds. Also complements protein disulfide-isomerase deficiency in yeast (By similarity). {ECO:0000250}.

Additionnal Information

Immunoblotting: use at 1ug/mL. A band of ~48kDa is detected. Positive control: heat-shocked HeLa cell lysate. These are recommended concentrations. Endusers should determine optimal concentrations for their applications.

Storage Conditions

This antibody is stable for at least one (1) year at -20°C.

Specificity

This antibody recognizes human EndoPDI.

Formulation

PBS, pH 7.4, 50% glycerol, 0.09% sodium azide.Purified by Protein G affinity chromatography.

Buffer pH

pH 7.4

Target Background

Endothelial protein disulfide isomerase (EndoPDI) is a thiredoxin member of the protein disulfide isomerase family of chaperones. This enzyme is localized in the endoplasmic reticulum in primary endothelial cells but colocalizes with nucleoli in the nuclei of breast, colon, and kidney cancer cells. EndoPDI mRNA and protein expression is iduced by hypoxia and exhibits a protective effect on endothelial cells during hypoxia.