Anti-LRP4 (extracellular) Antibody

Mouse Monoclonal Antibody specific to LRP4 (extracellular)

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

LRP-4, LDLR dan

Gene Name

Lrp4

NCBI Gene ID

UniProt

Q8VI56

Cellular Locus

Cell membrane, Single-pass type I membrane protein

Host

Mouse

Reactivity

Mouse, Rat

Immunogen

Fusion protein corresponding to aa 26-350 (extracellular N-terminus) of mouse LRP4 (accession no.Q8V156).

Target Antigen

Low-density lipoprotein receptor-related protein 4

Target

LRP4 (extracellular)

Clonality

Monoclonal

Isotype

IgG2a

Type

Antibody

Applications

WB, ICC/IF

Field of Research

Neuroscience

Purification Method

Purified by Protein G affinity chromatography

Concentration

1.0 mg/mL

Dilution

Dilute in PBS or medium that is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

Mediates SOST-dependent inhibition of bone formation. Functions as a specific facilitator of SOST-mediated inhibition of Wnt signaling. Plays a key role in the formation and the maintenance of the neuromuscular junction (NMJ), the synapse between motor neuron and skeletal muscle. Directly binds AGRIN and recruits it to the MUSK signaling complex. Mediates the AGRIN-induced phosphorylation of MUSK, the kinase of the complex. The activation of MUSK in myotubes induces the formation of NMJ by regulating different processes including the transcription of specific genes and the clustering of AChR in the postsynaptic membrane. Alternatively, may be involved in the negative regulation of the canonical Wnt signaling pathway, being able to antagonize the LRP6-mediated activation of this pathway. More generally, has been proposed to function as a cell surface endocytic receptor binding and internalizing extracellular ligands for degradation by lysosomes. Plays an essential role in the process of digit differentiation (PubMed:16517118). {PubMed:16517118, PubMed:18848351, PubMed:21471202}.

Additionnal Information

Immunoblotting: use at 1ug/mL. Predicted molecular weight is ~215kDa. Degradation products of approx. 150kDa are also detected. Positive control: rat brain lysate. These are recommended concentrations. Endusers should determine optimal concentrations for their applications.

Storage Conditions

This antibody is stable for at least one (1) year at -20°C.

Specificity

This antibody recognizes mouse and rat LRP4.

Formulation

PBS, pH 7.4, 50% glycerol, 0.09% sodium azide.Purified by Protein G affinity chromatography.

Buffer pH

pH 7.4

Target Background

The formation of neuromuscular junctions (NMJ) requires the interaction between motor neurons and muscle fibers. LRP4 (low-density lipoprotein receptor-related protein 4) is a receptor of agrin that is thought to act in cis to stimulate MuSK in muscle fibers for postsynaptic differentiation. Recent studies of muscle- specific mutants suggest that LRP4 is involved in determining where AChR clusters form in muscle fibers, postsynaptic differentiation, and axon terminal development.