V5 tag, AlpHcAbs® Mouse IgG2a
Anti-V5 tag, AlpHcAbs® Mouse IgG2a antibody is designed for detecting V5 tag fusion protein specifically. Anti-V5 tag, AlpHcAbs® Mouse IgG2a antibody is monovalent, recombinant single domain antibody fused to mouse IgG2a Fc. Based on western blot and ELISA, Anti-V5 tag, AlpHcAbs® Mouse IgG2a antibody reacts with the V5 tag sequence (GKPIPNPLLGLDST) selectively, no reactivity with other proteins.
Product Specifications
Host
Alpaca
Species Reactivity
Universal
Immunogen
V5 tag fusion protein
Conjugation
Non-conjugated
Type
Alpaca monoclonal Antibody
Applications
WB, ICC/IF, ELISA, IP, FC
Concentration
1 mg/mL
Dilution
WB: 1:10,000-1:50,000 ;ICC/IF: 1:200-1:1000 ;ELISA: 1:10,000-1:50,000 ;IP: 1-2ug/sample ;FC: 1µg for 106 cells
Purity
Recombinant Expression and Affinity purified
Form
Liquid
Buffer
Liquid, 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300, 50% Glycerol
Storage Conditions
Store at –20°C, (Avoid freeze / thaw cycles), Stable for 12 months at -20°C
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