Anti-Cytokeratin 18 Antibody

Mouse Monoclonal Antibody specific to Cytokeratin 18

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

Cell proliferation-inducing gene 46 protein, Cytokeratin-18, CK-18, Keratin-18, K18

Gene Name

KRT18

NCBI Gene ID

UniProt

P05783

Cellular Locus

Cytoplasm, perinuclear region. Nucleus, nucleolus.

Host

Mouse

Reactivity

Human

Immunogen

Recombinant human Cytokeratin 18.

Target Antigen

Keratin, type I cytoskeletal 18

Target

Cytokeratin 18

Clonality

Monoclonal

Isotype

IgG1

Type

Antibody

Applications

IHC

Field of Research

Cancer research

Purification Method

Purified by immunoaffinity chromatography

Concentration

Lot Specific

Dilution

Dilute in PBS or medium that is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Tris

Function

Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection. {PubMed:15529338, PubMed:16424149, PubMed:17213200, PubMed:7523419, PubMed:8522591, PubMed:9298992, PubMed:9524113}.

Additionnal Information

<br><br>Immunohistochemistry: use at a dilution of 1:100-1:200 on formalin-fixed, paraffin-embedded samples after heat-induced epitope retrieval at pH 9 for 10-30 minutes.

Storage Conditions

Store at 2-8°C. Do not freeze.

Specificity

Human Cytokeratin 18. Reactivity with other species has not been investigated.

Formulation

Tris buffer, pH 7.3-7.7, 1% BSA, 0.1% sodium azide.

Buffer pH

pH 7.3-7.7

Target Background

Cytokeratin 18 is a type I cytokeratin. Together with its filament partner cytokeratin 8, it is perhaps the most commonly found products of the intermediate filament gene family. They are expressed in single layer epithelial tissues. Mutations in this gene have been linked to cryptogenic cirrhosis. Multimers of cytokeratin 18 and cytokeratin 8 are present in simple, glandular, and transitional epithelial cells, but absent from stratified epithelial cells, and are found in adenocarcinomas of simple and glandular epithelium origin and poorly differentiated squamous carcinoma cells.