Glutamine Synthetase Inhibitor Screening Kit (Fluorometric)

Glutamine Synthetase (GS; EC 6.3.1.2) is a key enzyme that regulates nitrogen metabolism by catalyzing the formation of glutamine from glutamate and ammonia. It is ubiquitously expressed in humans and is linked to several neurological disorders including Alzheimer’s disease, schizophrenia, epilepsy etc. Inhibition of GS in animal models have shown significant therapeutic benefits in hepatic encephalopathy, amyotrophic lateral sclerosis, inflammatory liver failure, cancer and other human diseases. Additionally, GS is highly expressed in bacteria and is essential for its growth. Inhibition of Mycobacterium tuberculosis GS has been used as a therapeutic strategy against Tuberculosis. BioVision’s Glutamine Synthetase Inhibitor Screening Kit is a plate-based fluorometric assay for the screening human GS inhibitors. In this kit, the activity of GS is monitored by the formation of a fluorescent product formed via an enzymatic reaction and is detected at Ex/Em = 535/587 nm. But in the presence of GS inhibitors, the GS enzyme loses its activity, which results in the decrease of fluorescence intensity. Methionine Sulfoximine (MSO), a well-characterized inhibitor of prokaryotic and eukaryotic GSs with different Ki values is used as the positive control. The assay is simple and can used to identify and characterize human GS inhibitors.