Anti-GFRα-1 Antibody

Rabbit Polyclonal Antibody specific to GFR-1 (IN)

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

GDNF receptor α-1, GDNFR-α-1, GFR-α-1, RET ligand 1, TGF-beta-related neurotrophic factor receptor 1

Gene Name

GFRA1

Gene ID

2674

NCBI Gene ID

UniProt

P56159

Accession Number

NP_001138925.1

Cellular Locus

Cell membrane, Golgi apparatus, trans-Golgi network, Endosome, Endosome, multivesicular body

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Peptide corresponding to aa 369- 382 of human GFRa-1. This peptide sequence is identical to those of mouse and rat.

Target Antigen

GDNF family receptor α-1

Target

GFRα-1

Clonality

Polyclonal

Isotype

IgG

Type

Antibody

Applications

ELISA, WB, IHC-P, IF

Field of Research

Neuroscience

Purification Method

Purified by peptide immuno-affinity chromatography

Concentration

Lot Specific

Dilution

Dilute in PBS or medium which is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

Receptor for GDNF. Mediates the GDNF-induced autophosphorylation and activation of the RET receptor (By similarity). {ECO:0000250}.

Additionnal Information

Immunoblotting: use at 1:500 dilution.

Storage Conditions

This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles.

Specificity

This antibody recognizes human, mouse, and rat GFRalpha-1 (approx. 50 kD).

Formulation

PBS, pH 7.4.

Buffer pH

pH 7.4

Target Background

Glial cell line-derived neurotrophic factor (GDNF) is a potent survival factor for central and peripheral neurons and is essential for the development of the kidney and the enteric nervous system. Physiological responses to GDNF require the presence of a novel glycosylphosphatidylinositol linked protein, GDNFRalpha, a cell surface receptor for GDNF. The cDNAs encoding GDNFRalpha from human, rat, chicken, and mouse have been cloned. GDNFRalpha has also been referred to as GFRalpha-1, Ret ligand 1 (RETL1) and TGF- beta-related neurotrophic factor receptor 1 (TmR1). GFRalpha-1 binds GDNF specifically and mediates activation of the Ret protein tyrosine kinase (PTK). Thus, GDNF, GFRalpha, and the Ret PTK form a complex to transmit the GDNF signal and to mediate GDNF function. GFRalpha-1 has been identified in brain, liver, kidney, and spleen.