SARS-CoV-2 Spike S1 Protein (T95I, G142D, E154K, L452R, E484Q, D614G, P681R), His Tag

SARS-CoV-2 Spike S1, His Tag (S1N-C52Ht) is expressed from human 293 cells (HEK293) . It contains AA Val 16 - Arg 685 (Accession # QHD43416.1) . The mutations (T95I, G142D, E154K, L452R, E484Q, D614G, P681R) were identified in the SARS-CoV-2 Kappa variant (Pango lineage: B.1.617.1; other names: 21A/S:154K) .

Product Specifications

Background

It's been reported that Coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

Specifications

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 77.0 kDa. The protein migrates as 100-115 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Accession Number

QHD43416.1

Expression Region

Val 16 - Arg 685

Host

HEK293

Target

Spike S1

Conjugation

Unconjugated

Tag

C-10xHis

Source

SARS-CoV-2

Stability

● -20°C to -70°C for 12 months in lyophilized state; ● -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

95%

Bioactivity

Immobilized SARS-CoV-2 Spike S1, His Tag (Cat. No. S1N-C52Ht) at 2 μg/mL (100 μL/well) can bind Human ACE2, Fc Tag (Cat. No. AC2-H5257) with a linear range of 0.8-2 ng/mL (QC tested).